These challenges are partly due to the limited emission wavelength (less than 700 nm), undesirable mitochondrial targeting capabilities and the inadequate sensitivity of these sensors in detecting endogenous GSH levels, which typically range within the millimolar concentration
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Add reconstitution solvent slowly and directly onto the lyophilised powder, not against the vial wall
Translational success will depend on establishing standardized, benchmarked assays for ER and TrkB, supported by orthogonal phospho-proteomic verification to ensure inter-laboratory comparability
Jewell, J