Methylglyoxal comes of AGE
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Exogenous treatments with GSH or the synthetic cysteine precursor OTC caused a substantial increase in total glutathione (reduced GSH and oxidized GSSG), regardless whether or not the plants accumulate wild type levels of SA ( Figure 1A )
To further explore the substrate scope of arGSTs, additional yeast systems were engineered using a one-plasmid strategy containing genes encoding dihydroflavonol-4-reductase (DFR), LDOX, a range of arGST and non-arGSTs (as negative controls) and anthocyanidin-3- O -glucosyl transferase (A3GT) (Fig
MdxE-sugar binding assay The interaction of the purified MdxE with potential sugar substrates was determined by surface plasmon resonance using a Biacore T200 instrument (Cytiva) equilibrated at 25 C in 1 Phosphate-buffered saline (PBS, pH7.4) supplemented with 0.01% Tween20 and 100 M EDTA
