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Mechanistically, the peptide has been studied as a pro-angiogenic agent that appears to work through the VEGFR2 receptor and the endothelial nitric-oxide synthase (eNOS)nitric-oxide pathway, and as a stimulator of fibroblast outgrowth and migration through the FAKpaxillin signaling pathway

Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C

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