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2010 Mar 5;285(10):7097-110

BSO treatment, which inhibits the rate limiting enzyme for GSH synthesis, resulted in almost 80% reduction in extra mitochondrial GSH concentration compared to about 50% reduction in the mitochondrial GSH pool suggesting that cytosolic GSH is more sensitive to BSO compared to mitochondrial GSH

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The experiment requires dim lighting, and observers should maintain as much separation from the test animals as possible

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