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You do not titrate the dose up and down the way you might with a standalone peptide

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Maximum recommended dosage is twice a day

Following respirometry, wells which had no injections were washed twice with cold PBS before lysing with RIPA Lysis Buffer prepared in cold PBS with cOmplete, EDTA-free protease inhibitor (Roche, 11697498001) for 5 minutes

If a bubble enters your syringe, hold the syringe needle-up, tap the barrel to move the bubble to the top, and gently push it out before injecting