In the dimeric COP1-DDD-E2 complex, the WD40 domains of COP1 are orientated toward each other and separated by a distance of approximately 20
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After two more washes with PBS, biotin was stained with 1 g ml 1 streptavidin-Atto594 (Sigma-Aldrich), and nuclei were stained with NucBlue (Thermo Fisher Scientific, 1:200) in PBS for 60 min at room temperature
B12 is a water-soluble vitamin, so any excess quickly goes out through the urine
CD44 is a phagocytic receptor
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