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There are also a few examples of the use of other density barriers and two-layer discontinuous gradients, prepared from both Nycodenz and OptiPrep

Bauersfeld SP, Kessler CS, Wischnewsky M, et al

Step 4: Prepare the syringe Remove the needle and syringe from their wrappers

Gasparovic C, Song T, Devier D, Bockholt HJ, Caprihan A, Mullins PG, et al