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For metabolite detection, beads were reconstituted in 50 L of 80% methanol containing 20 M of [ 13 C 3 , 15 N]-NEM-Cysteine (Cambridge Isotope Laboratories, CNLM-38710H-0.25) and 40 M of [ 13 C 2 , 15 N]-NEM-GSH (Sigma-Aldrich, 683620), incubated on ice for 5 min, and extracts cleared by centrifugation at 17,000 g for 10 min

Retrieved August 24, 2023, from Ellison, D

Increased peripheral bloodneutrophil activation phenotypes and NETosis in critically ill COVID-19 patients

Acute inflammation is necessary for healing, but chronic inflammation delays recovery

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