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In detail, for TPC determination: 20 l extract (2.0 mg/mL) was mixed with 100 L Folin-Cioclteu reagent, and 80 L sodium carbonate (7.5% w/v ) ad incubated for 30 min at room temperature followed by measuring the absorbance at 760 nm using gallic acid as reference drug (0.0110.154 mg/mL, R 2 = 0.99)

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Missing doses, varying timing, or improper storage all reduce effectiveness