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As Content From Rice Tissues Rice root and shoot samples were washed with deionized water for approximately 5 min and incubated at 80C in an oven until they dried completely

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Insert the needle using a smooth, dart-like motion until it reaches the muscle

To quantify T-helper (CD4 + ) and cytotoxic (CD8 + ) T-cells a dual staining procedure was used (CD4: FITC, CC8, IgG2a and CD8a: RPE, CC63, IgG2a)

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The samples were then dehydrated using a graded sucrose solution (15 and 30% in ddHO) and subsequently embedded in paraffin