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The inclusion criteria for a food or ingredient to be considered functional involve food safety, free access without a prescription and evidence of health benefits when regularly consumed in a balanced diet (Granato et al., 2020)

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Molecular anatomy and functions of the choroidal blood-cerebrospinal fluid barrier in health and disease

The RNA was quantified using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, USA), and the RNA integrity was assessed using an Agilent 2100 system and RNA Nano 6000 Assay kit (Agilent Technologies, USA)