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These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six

Accordingly with previous studies (63, 64), animal responsiveness to forceps stimuli is tested with blunt-nosed thumb forceps with serrated tips for increased grip