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Inhibiting efflux pumps Efflux pumps play an important role in transporting substrates involved in various functions from bacterial cells to the outside, and they participate significantly in the development of antibiotic resistance 38,77

For the correlative light and electron microscopy studies, vibratome sections were cryoprotected with 2.3 M sucrose in 0.1 MPB and frozen in liquid nitrogen

Once trypomastigotes enter the blood stream of the vertebrate host, they quickly infect a variety of cells and differentiate to the replicative amastigote stage

Similar to LCLs, non-tumor epithelial and mesenchymal cells do not grow in these conditions, whereas a minor fraction of their tumor counterparts grows in these gels [26, 27]

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Immune system Since much of what Lyme disease is about is microorganisms, having a strong immune system is crucial