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While estrogen also regulates mitochondrial DNA transcription, mitochondrial morphology, and mitochondrial fission and fusion in breast cancer cells (reviewed in 203), these mechanisms are understudied in the context of immune cells

For each mouse, the percent of intestinal transit was calculated as the percentage of distance traveled by the charcoal meal relative to the total length of the small intestine

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The cell mixture was then placed in U-Shaped-Bottom Microplates (Thermo Fisher, Waltham, MA, USA) designed for spheroid formation

6 weeks: 3 vials use 6 mL total