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To assess mitochondrial morphology and functional states MitoTracker Green FM Dye, MitoTracker Red FM Dye, and TMRM, (all from Thermo Fisher Scientific) were used according to the manufacturer instructions

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The isolated cDNAs from the pool enriched in the fourth and fifth rounds were ligated into the pGEM-T-Easy vector (Promega), using DNA Ligation Kit Mighty Mix (Takara Bio), and cloned into DH5 competent cells

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[4] Separate investigations on human dermal papilla cell cultures have shown that related copper peptides can stimulate cell proliferation and reduce apoptotic signaling, findings that could translate into stronger follicular support in vivo