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(The supernatant can be stored at -80 for 10 days.) Cell sample Harvesting cell should not less than 106,then wash with PBS for twice (mix cell with PBS centrifuge at 600g for 10 minutes), mix precipitated cell with the volume of PBS for 3 times

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However, supplementation with [73] , and there were no benefits on appetite, weight, or quality of life in patients with gastrointestinal or lung tumors [74] , and it did not improve in patients with cancer in phase IV or palliative on fatigue, symptoms insomnia, appetite loss, depression, pain, and quality of life [76]

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[39] However, the American College of Cardiology supported the trial

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