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In addition, deletion of the GSH1 geneencoding glutamylcysteine synthetase (GCS), the enzyme catalysing the first step in glutathione synthesiswas shown to suppress an Ero1 conditional mutant, again indicating that GSH provides an additional load on Ero1 activity (Frand & Kaiser, 1998)

L., & Varady, K

After different treatment conditions, the supernatants of HCM were collected for ELISA using CoQ10 and CKMB Enzyme Immunoassay (EIA) kits (Clond-Clone, China), respectively

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