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Once this mobilization is completed, BECs return to a quiescent-like state that is associated with the formation of cellcell junctions and vessel maturation (200)

The T7 promotor in the control plasmid should not lead to protein expression in mammalian cells

Perrone A, Giovino A, Benny J, Martinelli F (2020) Advanced glycation end products (AGEs): biochemistry, signaling, analytical methods, and epigenetic effects

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thus, the major risk factor is family history