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Figure 3 Table 3 Gut dysbiosis affect hypothalamus (gut-liver-hypothalamus axis)

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Lockrow J, Prakasam A, Huang P, Bimonte-Nelson H, Sambamurti K, Granholm AC (2009) Cholinergic degeneration and memory loss delayed by vitamin E in a Down syndrome mouse model

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The digested samples were then subjected to LCMS/MS analysis using a ShimazuLC (LC-20AB pump) system coupled to a 4000 QTRAP triple-quadrupole MS instrument (AB Sciex) and a C18 column (250 mm 2.1 mm inner diameter, 3-m particle size