Insert the needle into the rubber membrane and inject the air from the syringe into the vial
BAC water added 1.0 mL Concentration 5 mg/mL (5,000 mcg/mL) 300 mcg dose 0.06 mL / 6 units 500 mcg dose 0.10 mL / 10 units BAC water added 2.0 mL Concentration 2.5 mg/mL (2,500 mcg/mL) 300 mcg dose 0.12 mL / 12 units 500 mcg dose 0.20 mL / 20 units BAC water added 3.0 mL (recommended for accuracy) Concentration ~1.667 mg/mL (1,667 mcg/mL) 300 mcg dose 0.18 mL / 18 units 500 mcg dose 0.30 mL / 30 units 3.0 mL is the most common research-context dilution because it pushes daily draws above 10 syringe units, which makes measurement on a 0.3 mL U-100 syringe easier
The local climate, characterized by intense sunlight, can also impact B12 absorption
What they did: Isolated human chondrocytes (from osteoarthritis patients and healthy donors) were exposed to AOD-9604 at doses 1 to 1000 nM
But, a doctor should decide the best one for you
Assemble Your Injection Device: If you're using a syringe and vial, remove the caps and, using a new needle, draw air into the syringe equal to your dose