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Used when active site is located deep within a sample molecule If too long, it can interact with sample species on its own (London interactions) If too short, ligand cant reach active site on sample molecule

Such a setup would precisely delineate the NSC-derived paracrine factors (e.g., BDNF, VEGF [18, 32], TGF-) responsible for suppressing microglial activation and promoting an anti-inflammatory phenotype, independently of in vivo complexities

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