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For colony formation assays, cells (5 10 4 ) were suspended in 2 mL of 0.33% noble agar (Difco) and laid over 6-well culture plates previously coated with 2 mL of 0.5% noble agar

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Data indicates GHK-Cu synergizes with physical and chemical methods

Independently tested

- Concentracin final 0.1%0.5%

Reduced expression of PGC-1 partly underlies mitochondrial changes and correlates with neuronal loss in multiple sclerosis cortex