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Trypan blue for cell death and DAB staining for H 2 O 2 accumulation Detached leaves were immersed in trypan blue staining solution (2.5 mg ml 1 trypan blue dissolved in lactophenol:lactic acid:glycerol:liquid phenol:ddH 2 O at 1:1:1:1:1) for 30 min

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The result of this study showed that lupus PBLs have increased oxygen consumption through mitochondrial ETC complex I, which is the main source of oxidative stress in SLE, and this complex is inhibited by NAC [22]