Antibodies Specific primary antibodies: Akt (2532 S, 1:2000), pS473-Akt (9271 S, 1:1000), S6K (9202 S, 1:2000), pT389-S6K (9205 S, 1:1000), Stat5 (9420 S, 1:10000), and pY694-Stat5 (9356 S, 1:1000) were obtained from Cell Signaling
Here's how to do it seamlessly: Schedule Your Injections : Opt for a specific time each week for your injections to create a consistent routine
Invest 111 , 15191527 (2003)
The nitrocellulose membranes were immunoblotted with primary antibodies against acyl-CoA synthetase long-chain family member 4 (ACSL4) (1:1000, Santa cruz), Bax (1:1000, Cell signaling), Bcl-2 (1:1000, Cell signaling), DRP1 (1:1000, Cell signaling), GAPDH (1:5000 Abcam), GPX4 (1:1000, Abcam), MLKL (1:1000, Invitrogen), OPA1 (1:1000, Cell signaling), pDRP1 (Ser616) (1:1000, Cell signaling), pRIPK3 (Ser232) (1:1000, Cell signaling), and RIPK3 (1:1000, Cell signaling) overnight at 4 C, and then incubated with the appropriate secondary antibody (1:1000, Santa cruz)
Although siRNA studies show that GSTO1 is important for HCT116 cancer cell viability, knockdown of GSTO1 did not appear to affect C1-27 cytotoxicity, suggesting that other targets of C1-27 also may contribute to its cytotoxicity and have a compensatory effect (Supplementary Fig
