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Date: 1 November 2022) and all experiments were performed in accordance with institutional guidelines for the care and use of laboratory animals

Quantitative RT-PCR On day 32 post-airlift, three replicate ALI cultures from each KO treatment (5 donors) were washed for 5 min with 1 PBS at 37 C before direct lysis on the transwell insert

However, it is to be determined whether this proposed rule will be subject to further litigation

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The captain raised his hand, calling for a halt