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However, the TSNA levels detected with the MIP-HPLC column were within the same reported ranges as TSNAs in e-liquids detected with traditional HPLC columns

Phan TTP (2015) Intracellular expression and investigation of the possibility for purifying recombinant protein in Bacillus subtilis using reporter GFP

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Bacterial pellets were sonicated at 70% amplitude, 2 s on5 s off for 5 min on ice in lysis buffer (20 mM Tris-HCl, pH 7.2, 500 mM NaCl, 10 mM imidazole, 5% glycerol, 0.5 mg ml 1 lysozyme (Thermo, 89833), 0.5% octyl-B-D-thioglucopyranoside (CHEM-IMPEX, 21810), 2 mM PMSF (Roche) and 2 mM -mercaptoethanol (Sigma, M3148), and the pH was adjusted to 8.0 with NaOH), after which, Benzonase (~25 U ml 1 ), MgCl 2 (1 mM) and CaCl 2 (1 mM) were added and the lysate was incubated on ice for 15 min