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Plasmids were transfected using Lipofectamine 2000 (Invitrogen) according to the product protocol

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While serious adverse events (SAEs) or other problems are not anticipated, serious and unanticipated and related adverse events or unanticipated problems involving risks to participants or others will be reported within 48 h to the Yale IRB and within 72 h to the relevant Serious Adverse Event Tracking and Reporting System at NIDA/NIH