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Paraffin blocks were sectioned onto SuperFrost Plus slides, dried overnight, and baked for 1 h at 60 C before immediately proceeding with the RNAScope Multiplex Fluorescent v2 assay according to the manufacturers instructions with the following modifications: Target retrieval was performed for 15 min in a boiling beaker, Protease III was used for 30 min pre-treatment at 40 C and hybridized slides were left overnight in 5 SSC before proceeding with the amplification and labeling steps
101 st Congress ASTM Standard E2187
25 One of the options considered by NASA officials in early 1963 was to fly another Mercury-Atlas spacecraft in order to increase U.S
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Braking is by the largest automotive braking system in the world 440 mm Carbon-Silicon-Carbide (CSiC) discs on the front axle, clamped by 10-piston calipers, and 410 mm discs with four-piston calipers at the rear fitted as standard
