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3.4 Metabolic profiling To investigate the impact of different fermentation times on metabolites, a comprehensive metabolic profiling was conducted on samples T0, T1, T2, and T3 using UPLC-MS/MS, and a total of 1801 metabolites were identified, including alkaloids (15.99%), terpenoids (13.27%), amino acid and its derivatives (12.1%), flavonoids (9.88%), lipids (9.49%), phenolic acids (9.38%), organic acid (8.11%), nucleotide and its derivatives (3.83%), lignans and coumarins (3.22%), quinones (1.17%), steroids (0.28%), tannins (0.06%), and others (13.21%) (Figure 4A)

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