Quantitative real-time PCR of DAB2IP transcript was carried out on StepOnePlus Real-time PCR System (Applied Biosystems, Foster City, CA, USA) using specific FAM-labelled probe and primers (revalidated TaqMan Gene expression assay for DAB2IP Hs00368995, Assays on Demand, Applied Biosystems)
If youre an asshole, Im gonna be like: Yo, fuck off. You dont get to talk to her like that, she says: Im confrontational. Confrontational and mega-famous pop star make for an uneasy combo (for her publicist, at least)
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