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Calibration standards 47 were prepared by adding 0.05, 0.1, 0.25, and 0.5 mL of intermediate nicotine degradants and impurities standard solution 1 (100 g/mL) to separate 10 mL volumetric flasks and diluting to volume with a methanol/Type 1 water (70:30, v/v) solution

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users inhale the vapor that is produced

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