To get a better picture of the health effects of light smoking, researchers tracked more than 290,000 adults aged 59 to 82, including more than 22,000 current smokers and more than 156,000 former smokers, who completed surveys in 2004 and 2005
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After resuspending bacterial cells in the lysis buffer supplemented with 10 mM imidazole, proteins were captured with the Ni-NTA affinity chromatography (Sigma-Aldrich) and subjected to SEC in buffer A
