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Multivariate logistic regression analysis was performed using daily smoking as the dependent variable and age, sex, race/ethnicity, rurality, age when first tried cigarette smoking, smoking at school in the past 30 days, and using smokeless tobacco as the independent co-variates (Table 4)

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These pieces were incubated in Ca/Mg-free Hanks balanced salt solution (HBSS, SigmaAldrich) containing 30 mM ethylenediaminetetraacetic acid (EDTA, SigmaAldrich) and 1.5 mM dithiothreitol (DTT, SigmaAldrich) at 37 C with shaking for three cycles of 10 min each

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Many individuals view it as a less harmful alternative, particularly for those trying to quit smoking or reduce their nicotine intake