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After incubation, reactions were quenched by addition of 150 l per well colour solution (2:1 antipyrine/H 2 SO 4 : oxime reagents, prepared immediately before use), sealing the plate with plate film (Thermo), pulse vortexing and heating on top of a 95 C heat block for ~30 min to 1 h in room light

Lubrication: Apply food-safe oil to metal moving parts as recommended by the manufacturer to ensure smooth operation

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"With California having such a huge population, it's going to be very impactful nationwide," said Cathy Callaway, associate director of state and local campaigns for the society