Effect of CSC on C albicans adhesion A glass slide (0.5 cm in diameter) was placed into each well of a 12-well plate then covered with 1 ml of sterile artificial saliva solution at room temperature for 30 min under agitation to ensure that the slides were covered by liquid at all times
siRNA Transfection in vitro When HBE cells reached 3050% confluence, they were transfected with SREBP2-siRNA (sc-36559, SantaCruzBiotechnology, USA), HMGCR-siRNA (sc-44851, SantaCruzBiotechnology, USA), ABCA1-siRNA (sc-41137, SantaCruzBiotechnology, USA) or Control-siRNA (sc-37007, SantaCruzBiotechnology, USA) at 10 nM
Research data were stored on password-protected electronic devices
An editorial accompanying the study and co-written by Borrelli recommended e-cigarettes only after smokers have tried and failed to quit with FDA-approved products
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Vaping is a popular alternative among people trying to quit smoking, as it allows users to control their nicotine intake while mimicking the physical and sensory aspects of smoking