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The flask was placed in an incubator at a temperature of 37 C with 5% CO 2 for 48 h

In addition, individuals who smoke conventional cigarettes and use e-cigarettes (dual use) showed the highest likelihood of developing COPD compared with those who have never smoked conventional cigarettes or used e-cigarettes [108, 110,111,112]

When cigars are stored in appropriate conditions with controlled humidity and temperature, the nicotine content tends to remain consistent

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We performed comprehensive bioinformatics analysis and experimental validation to discover the expression of NtPSY under different abiotic stresses, and investigated the functions of NtPSY1 and NtPSY3 by transient silencing experiments