After washing twice in 1 PBS, cells were resuspended in ALI expansion medium with RI and seeded onto bovine collagen-coated 6.5 mm transwell inserts (2 10 4 cells/insert) containing ALI Expansion medium with RI in the basolateral chamber (day 5)
Available from: www.kff.org 81 Smoking and Diabetes
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Then you can move on to designing the diagram
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I've heard that Amber is on it's way out as well, but I think you can still find it online pretty easily at this point
