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The culture conditions of isolated strains were as follows: LB medium (5.0 g/L yeast extract, 10.0 g/L tryptone, and 10.0 g/L NaCl, pH 7.0, sterilized at 115 for 30 min), potato dextrose agar (PDA) medium (200.0 g/L potato was cut into small pieces, boiled for 2030 min, and filtered with 8 layers of gauze), and glucose 20.0 g/L, agar 20.0 g/L, natural pH

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Langton, in Brockman, The Third Culture (1995), excerpted at Edge.org, Chapter 21: A Dynamical Pattern (1996) 411In the late nineteenth century, the Austrian physicist Ludwig Boltzmann showed that one could account for many of the thermodynamic properties of macroscopic systems in terms of the collective activity of their constituent atoms

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