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Liquid chromatography and mass spectrometry conditions Analyses of urinary nicotine, cotinine and NNAL were conducted using an HPLC (UltiMate 3000, Thermo Fisher Scientific, Yokohama, Japan) coupled to a hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific Q Exactive Focus) with heated electrospray ionization (HESI-II)

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In this study, the average NNAL levels of the subjects at the initial visit to the clinic were similar to those of the active smokers in the previous report