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Following centrifugation at 500 g for 20 min at 21 C, the buffy coat was collected, washed twice with PBS, centrifuged again, and prepared for flow cytometry staining

Among Big Stars outspoken admirers were the bands R.E.M., Wilco, and the Replacements, who even wrote a song called Alex Chilton. Big Star might have finally broken out of its cult niche last year with the release of a four-CD box set called Keep An Eye on the Sky

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The selection was based on the availability of recently manufactured and packed samples, which were randomly purchased from local retail outlets

Travellers angry at duty free changes, said the headline in national newspaper The Age