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PMID: 36014491 Tymoshenko, M

Supplementary Table 1) was mixed with 3 l of transfection reagent (DharmaFECT 1, Horizon Discovery) in a total volume of 500 l of OptiMEM (Gibco) and incubated for 20 minutes at room temperature

Under hypoxic conditions, the mitochondrial Ala-tRNA synthetase AARS2 catalyzes lactylation of PDHA1 at lysine 336, blocking pyruvate entry into oxidative phosphorylation and redirecting carbon flux toward glycolysis, thereby accelerating the Warburg metabolic program [55]