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Total RNA from biological replicates was extracted using the RNeasy Mini Kit (74104, Qiagen) according to the manufacturers instructions and analyzed by whole transcriptomic sequencing (Psomagen Inc), which returned RSEM counts and effective gene sizes

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There was something impossibly romantic about cinema, and Bogart was its poster boy, yet not in a conventional sense

According to the Centers for Disease Control and Prevention, smoking: Causes thickening and narrowing of blood vessels Raises triglycerides (a type of fat in your blood) Lowers HDL or "good" cholesterol Makes blood sticky and more likely to clot, which can block blood flow to the heart and brain Damages the cells that line the blood vessels

The FDA would like to limit how much can be present in cigarettes and combusted tobacco products

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