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The elution gradient was as follows: the initial conditions were 97% A:3% B at time 0, followed by a linear gradient to 15% A:85% B at 12.00 min, then a gradient to 3% A:97% B at 12.10 min, maintained at 3% A:97% B until 14.00 min, returned to the initial conditions of 97% A:3% B at 14.10 min, and then held until 16.00 min to reequilibrate the column

This is known lactam-lactim tautomer

Appendix 14.1, online at www.surgeongeneral.gov, provides a more complete discussion of this topic

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The difference between Oriental and Virginia tobacco leaves Both Oriental and Virginia tobaccos have unique characteristics that make them suitable for different uses

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