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Method of metabolomics detection and analysis For lung tissue homogenate samples, 400 l MeOH (containing 100 ng/mL Warfarin as Internal Standard) was added to the samples (100 l), which were then vortexed for 60 s, followed by centrifugation at 14,000 g and 4 for 15 min
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Generally speaking: Heavy vaping is around 80100 mL per month ( 2.73.3 mL/day) Moderate vaping is between 5080 mL per month ( 1.72.7 mL/day) Light vaping is around 2040 mL per month ( 0.71.3 mL/day) 5
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