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The test item design considered three points to address confounding factors: (1) the same tobacco blend structure was used for THS 2.2 and comparator cigarette test items, (2) tobacco from the same crops was used to exclude possible differences due to agricultural variability, and (3) no flavors were added to the test items

Bacterial odours, often the most pungent, result from factors such as dogs in cars, spilt milk, vomit, or ground-in food around the back seats

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In vitro cell experiments also confirmed that miR-34a mimic and Notch1 gene plasmids were transfected into HPMECs exposed to CSE