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Sperm DNA is also known to get damaged by exposure to tobacco smoke

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DISCUSSION This study showed that outdoor smoking causes detectable airborne nicotine and 3-EP levels in public outdoor spaces

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Its less of a health problem going forward

A goat anti-chicken AlexaFluor 488 secondary antibody (711-225-152, Jackson ImmunoResearch) at 1:1000 dilution was then used in a solution of 1.5% BSA in PBS