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A recent study reported that cigarette smoke exposure could enhance the expression of inducible nitric oxide synthase (iNOS) and activate nod-like receptor family pyrin domain containing 3 (NLRP3) inflammasome, both of which contributed to endothelial injury and vascular dysfunction

Reaction conditions were 95 C for 2 minutes, followed by 40 cycles of 95 C for 15 seconds, 60 C for 1 minute