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Cells were cultured in basic Alpha Medium (Corning, New York, USA) with 10% fetal bovine serum (Biowest, Riverside, MO, USA), 20 mM HEPES (Corning, New York, USA) and 1% penicillin-streptomycin (Gibco, Waltham, MA, USA)

They were developed by a Swedish company called Niconovumled by celebrated nicotine and smoking cessation expert Karl Olov Fagerstrmin the early 21st century

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In contrast, other cells of the body suffered only indirect damage, as tobacco smoking seems to affect key mechanisms in these cells that in turn mutate DNA. Joint lead author of the study from the Wellcome Trust Sanger Institute says, Our research indicates that the way tobacco smoking causes cancer is more complex than we thought

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