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When human lung epithelial cells (BEAS-2B and H292) were exposed to this aerosol for 1 h, a marked cytotoxicity was observed in BEAS-2B but not in H292 cells, 24 h later

Study protocol and data collection We retrieved the participants basic characteristics such as gender, age, pack-years (packs per day years), the number of daily cigarettes, and the Fagerstrm test of nicotine dependence (FTND) score 23 , using a questionnaire administered by trained nurses, during the participants first smoking cessation outpatient clinic visit

People also learn tips to help prevent weight gain and identify the best techniques for dealing with a smoking addiction

The Thermocycler (C1000 Thermal cycler, Bio-Rad, USA) was used following this program: Initial denaturation at 95 C for 3 min followed by 35 cycles of denaturation at 95 C for 30 s

The method used for quitting smoking may also be important

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