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Cigarette smoke exposure HBE cells were exposed to mainstream smoke from 3R4F University of Kentucky reference cigarettes (Lexington, KY) using a VC-10 Smoking Robot (Vitrocell, Waldkirch, Germany) in accordance with ISO 3308 standards (puff duration 2 s, hold time 0 s, exhaust time 8 s, puff volume 35 mL, frequency 60 s, 6 puffs per cigarette)

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Some people prefer this type of machine because theyre less expensive than electric versions, while others just like the idea of having more control over their product when using an electric device

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This only shows the theoretical nicotine content per puff in the liquid, not actual absorption

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